Another paper on cAMP, crp, and adenylate cyclases in Shewanella

Here is a very recent publication in J. Bact.  The authors show that Shewanella oneidensis MR-1 has two adenylate cyclases that are essential to anaerobic respiraiton in Shewanella.  These are the same cya’s as shown in the arsenate respiring Shewanella, strain ANA-3.  The authors also report on transcriptome analysis of crp and adenylate cyclase mutants.  This is a solid paper with interesting results.  Here is the Pubmed link:

Charania et al. 2009. Involvement of a membrane-bound class III adenylate cyclase in the regulation of anaerobic respiration in Shewanella oneidensis MR-1 J. Bacteriology, Apr 24. Epub ahead of print.

 

What’s new for Shewanella

It’s been a long time since my last post.  A lot has happened since then in the land of Shewanella.  I’ve included a couple of links to recent Shewanella publications.  One in particular is the identification of adenylate cyclases that are involved in anaerobic respiration and showing that cAMP-CRP regulates arsenate respiration pathway.  The work was done in Shewanella sp. ANA-3, which is slightly different than MR-1; ANA-3 can also respire arsenate.  The other paper that might be of interest to Shewanella folks is the use of proteomics to characterize a regulator in MR-1 that might be involved in chromate transformation.

Comparative Temporal Proteomics of a Response Regulator (SO2426)-Deficient Strain and Wild-Type Shewanella oneidensis MR-1 During Chromate Transformation.
Chourey K, Thompson MR, Shah M, Zhang B, Verberkmoes NC, Thompson DK, Hettich RL.
J Proteome Res. 2009 Jan 2;8(1):59-71.
PMID: 19118451 [PubMed - as supplied by publisher]

Functional roles of arcA, etrA, cyclic AMP (cAMP)-cAMP receptor protein, and cya in the arsenate respiration pathway in Shewanella sp. strain ANA-3.
Murphy JN, Durbin KJ, Saltikov CW.
J Bacteriol. 2009 Feb;191(3):1035-43. Epub 2008 Dec 5.
PMID: 19060154 [PubMed - in process]

 

New Shewanella papers

Hello Shewanella folks,

Here are a couple of new papers from the Shewanella Fed. I haven’t thoroughly read them yet, but there are some interesting findings nestled in each paper.

1.  McLean JS, Pinchuk GE, Geydebrekht OV, Bilskis CL, Zakrajsek BA, Hill EA, Saffarini DA, Romine MF, Gorby YA, Fredrickson JK, Beliaev AS.
Oxygen-dependent autoaggregation in Shewanella oneidensis MR-1.
Environ Microbiol. 2008 Apr 9 PMID: 18412550

2: Yang Y, Harris DP, Luo F, Wu L, Parsons AB, Palumbo AV, Zhou J.
Characterization of the Shewanella oneidensis Fur gene: roles in iron and acid
tolerance response.
BMC Genomics. 2008;9 Suppl 1:S11. PMID: 18366600

Happy reading!