Here is a very recent publication in J. Bact. The authors show that Shewanella oneidensis MR-1 has two adenylate cyclases that are essential to anaerobic respiraiton in Shewanella. These are the same cya’s as shown in the arsenate respiring Shewanella, strain ANA-3. The authors also report on transcriptome analysis of crp and adenylate cyclase mutants. This is a solid paper with interesting results. Here is the Pubmed link:
May 5, 2009
Another paper on cAMP, crp, and adenylate cyclases in Shewanella
Posted by shewanella under General, Regulation[4] Comments
May 6, 2009 at 9:14 am
Whats up with this; are they calling “Ars” as “Art” and saying it is reversible? I don’t have time to read the paper but is there still ambiguity in the naming of these As reductases?!
“Respiratory arsenate reductase as a bidirectional enzyme” -oremland & Stolz
Abstract: The haloalkaliphilic bacterium Alkalilimnicola ehrlichii is capable of anaerobic chemolithoautotrophic growth by coupling the oxidation of arsenite (As(III)) to the reduction of nitrate and carbon dioxide. Analysis of its complete genome indicates that it lacks a conventional arsenite oxidase (Aox), but instead possesses two operons that each encode a putative respiratory arsenate reductase (Art). Here we show that one homolog is expressed under chemolithoautotrophic conditions and exhibits both arsenite oxidase and arsenate reductase activity. We also demonstrate that Art from two arsenate respiring bacteria, Alkaliphilus oremlandii and Shewanella sp. strain ANA-3, is also biochemically reversible. Thus Art can function as a reductase or oxidase. Its physiological role in a specific organism, however, may depend on the electron potentials of the molybdenum center and [Fe-S] clusters, additional subunits, or constitution of the electron transfer chain. This versatility further underscores the ubiquity and antiquity of microbial arsenic metabolism. (C) 2009 Elsevier Inc. All rights reserved.
May 6, 2009 at 9:18 am
Sorry, I meant are they confusing “Arr”- the dissimilatory reductase vs. “Art”
You guys fathered the “Arr” pathway; if they are referring to the same thing as “Art” that is a heated issue! Doubt they would tho since they are outstanding scientists.
May 6, 2009 at 9:21 am
Good eye on catching the typo. I’m sure they meant Arr. $#% happens, so I’d cut them some slack. There are typo errors in some of my papers too.
August 15, 2009 at 1:04 am
better get leave women for themselves and get back in the lab. Love you Chad.