From the Shewanella gurus. AFM analysis shows that MR-1 has an outer coating of decaheme cytochromes. Pretty neat stuff.
I’ve got a crazy idea… attach the lipid signal peptide of OmcA or MtrC to something like CymA and see if you can send it to the outer membrane.
Appl Environ Microbiol. 2009 Mar 13. [Epub ahead of print]
Antibody-recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1.
Lower BH, Yongsunthon R, Shi L, Wildling L, Gruber HJ, Wigginton NS, Reardon CL,
Pinchuk GE, Droubay TC, Boily JF, Lower SK.
Antibody-recognition force microscopy showed that OmcA and MtrC are expressed on
the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III),
including solid phase hematite (Fe2O3), was the terminal electron acceptor. OmcA
was localized to the interface between the cell and mineral. MtrC displayed a
more uniform distribution across the cell surface. Both cytochromes were
associated with an extracellular polymeric substance.
PMID: 19286784 [PubMed - as supplied by publisher]
