It’s been a long time since my last post. A lot has happened since then in the land of Shewanella. I’ve included a couple of links to recent Shewanella publications. One in particular is the identification of adenylate cyclases that are involved in anaerobic respiration and showing that cAMP-CRP regulates arsenate respiration pathway. The work was done in Shewanella sp. ANA-3, which is slightly different than MR-1; ANA-3 can also respire arsenate. The other paper that might be of interest to Shewanella folks is the use of proteomics to characterize a regulator in MR-1 that might be involved in chromate transformation.
Comparative Temporal Proteomics of a Response Regulator (SO2426)-Deficient Strain and Wild-Type Shewanella oneidensis MR-1 During Chromate Transformation.
Chourey K, Thompson MR, Shah M, Zhang B, Verberkmoes NC, Thompson DK, Hettich RL.
J Proteome Res. 2009 Jan 2;8(1):59-71.
PMID: 19118451 [PubMed - as supplied by publisher]
Functional roles of arcA, etrA, cyclic AMP (cAMP)-cAMP receptor protein, and cya in the arsenate respiration pathway in Shewanella sp. strain ANA-3.
Murphy JN, Durbin KJ, Saltikov CW.
J Bacteriol. 2009 Feb;191(3):1035-43. Epub 2008 Dec 5.
PMID: 19060154 [PubMed - in process]
February 26, 2009 at 9:59 pm
Hello,
I am not your lab colleague. But I am also working on Shewanella. I visit this blog from time to time. Hope you guys don’t mind ^_^
Here is another recent Shewanella publication which might be interesting.
Genomic reconstruction of Shewanella oneidensis MR-1 metabolism reveals a previously uncharacterized machinery for lactate utilization
PNAS February 24, 2009 vol. 106 no. 8 2874-2879
http://www.pnas.org/content/106/8/2874
March 3, 2009 at 8:05 am
First blog I read after wakeup from sleep today!
—————————-
March 20, 2009 at 1:46 am
Darn, you already have the blog I want.
Better keep it up or I’ll apply for As funding!
Need a postdoc? :)
March 20, 2009 at 1:48 am
ANA3 respires strain 200’s wake!
Sorry couldn’t resist.
March 20, 2009 at 3:26 am
Go for it! Write me in some funds too.
March 20, 2009 at 3:36 am
I’ve never tried 200. CN-32 and W3-18-1 are not too bad either. But, ANA-3 I think is the quickest.
March 20, 2009 at 3:52 am
10^4 cells/mL strain 200 respires 12 mM HFO in 24 hours when primed for growth on fumarate! No decimal error – 10^4!
yeah yeah so ANA3 & CN32 may be faster but those extra Arr proteins would weigh them down in an open subsurface race!
March 20, 2009 at 5:37 am
Strain 200 must have some crazy nanowires. And don’t forget the flavins…
April 22, 2009 at 3:44 pm
I love your blog. i am working on MFC and very interested in metal reducing bacteria like Shewanella. Will keep visiting your blog, thanks:)
April 23, 2009 at 4:13 am
Thanks. I’ve been a little slow to posting new findings on Shewanella. MFCs should be a great tool to study microbial reduction of solids such as minerals. Seems like much of the science is focused on the MFC design and less on the microbe. That’s changing however. Good luck with your work and I’ll try to update the blog more often.
Regards,
C