Here are a couple of Shewanella papers that I’ve got on my “to read list”:
Title: Direct involvement of type II secretion system in extracellular translocation of Shewanella oneidensis outer membrane cytochromes MtrC and OmcA
Author(s): Shi, L; Deng, S; Marshall, MJ; Wang, ZM; Kennedy, DW; Dohnalkova, AC; Mottaz, HM; Hill, EA; Gorby, YA; Beliaev, AS; Richardson, DJ; Zachara, JM; Fredrickson, JK
Source: JOURNAL OF BACTERIOLOGY 190 (15):5512-5516 2008
Abstract: MtrC and OmcA are cell surface-exposed lipoproteins important for reducing solid metal oxides. Deletions of type II secretion system (T2SS) genes reduced their extracellular release and their accessibility to the proteinase K treatment, demonstrating the direct involvement of T2SS in translocation of MtrC and OmcA to the bacterial cell surface.
Title: Expression of chromate resistance genes from Shewanella sp strain ANA-3 in Escherichia coli
Author(s): Aguilar-Barajas, E; Paluscio, E; Cervantes, C; Rensing, C
Source: FEMS MICROBIOLOGY LETTERS 285 (1):97-100 2008
Abstract: The plasmidic chromate resistance genes chrBAC from Shewanella sp. strain ANA-3 were transferred to Escherichia coli. Expression of chrA alone, on a high- or low-copy number plasmid, conferred increased chromate resistance. In contrast, expression of the complete operon chrBAC on a high-copy number plasmid did not result in a significant increase in resistance, although expression on a low-copy number plasmid made the cells up to 10-fold more resistant to chromate. The chrA gene also conferred increased chromate resistance when expressed in Pseudomonas aeruginosa. The chrR gene from the P. aeruginosa chromosome was necessary for full chromate resistance conferred by chrA. A diminished chromate uptake in cells expressing the chrA gene suggests that chromate resistance is due to chromate efflux.
